EFEKTIVITAS PENAMBAHAN MIKORIZA DAN EKSTRAK TOMAT PADA MEDIA AKLIMATISASITERHADAP PERTUMBUHAN PLANLET PISANG (Musa.sp)

Siti Mahardhika, Sari and ACHMAD, FATCHUL AZIEZ and Endang Suprapti, - and Tyas Soemarah K.D., - and Daryanti, Daryanti and Wiyono, - (2025) EFEKTIVITAS PENAMBAHAN MIKORIZA DAN EKSTRAK TOMAT PADA MEDIA AKLIMATISASITERHADAP PERTUMBUHAN PLANLET PISANG (Musa.sp). Jurnal Ilmiah Hijau Cendekia.

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Abstract

ABSTRAK Aklimatisasi merupakan tahap kritis dalam perbanyakan tanaman secara in vitro karena planlet harus beradaptasi dari kondisi steril ke lingkungan eksternal yang lebih bervariasi, sehingga rentan terhadap stres lingkungan. Penelitian ini bertujuan untuk mengevaluasi efektivitas penambahan mikoriza dan ekstrak tomat pada media aklimatisasi terhadap pertumbuhan planlet pisang (Musa sp.) hasil kultur jaringan. Penelitian dilaksanakan menggunakan Rancangan Acak Kelompok (RAK) dua faktor, yaitu dosis mikoriza (0 g/tanaman, 5 g/tanaman, 10 g/tanaman) dan konsentrasi ekstrak tomat (0%, 5%, 10%) dengan sembilan kombinasi perlakuan. Parameter pengamatan meliputi persentase bibit hidup, tinggi tanaman, diameter pangkal batang, dan jumlah daun aktif. Hasil penelitian menunjukkan bahwa kombinasi mikoriza dan ekstrak tomat berpengaruh nyata terhadap seluruh parameter pertumbuhan. Perlakuan tertinggi (M2E2) menghasilkan persentase hidup 99.2%, tinggi tanaman 27.2 cm, diameter pangkal batang 0.95 cm dan jumlah daun aktif 7 helai. Peningkatan ini menunjukkan bahwa mikoriza berperan dalam memperluas serapan hara dan memperkuat sistem perakaran, sedangkan ekstrak tomat menyediakan hormon pertumbuhan dan antioksidan yang mendukung proses adaptasi. Temuan ini menegaskan bahwa kombinasi mikoriza 10 g/tanaman dan ekstrak tomat 10% merupakan perlakuan terbaik untuk meningkatkan keberhasilan aklimatisasi planlet pisang secara efektif, efisien, dan ramah lingkungan, serta berpotensi diterapkan pada produksi bibit hortikultura lainnya. Kata Kunci : Mikoriza, ekstrak tomat, aklimatisasi, kultur jaringan, planlet pisang. ABSTRACT Acclimatization is a critical stage in in vitro plant propagation because plantlets must adapt from sterile conditions to a more varied external environment, making them vulnerable to environmental stress. This study aimed to evaluate the effectiveness of adding mycorrhizae and tomato extract to the acclimatization medium on the growth of banana plantlets (Musa sp.) from tissue culture. The study was conducted using a two-factor Randomized Block Design (RBD), namely the dose of mycorrhizae (0 g/plant, 5 g/plant, 10 g/plant) and the concentration of tomato extract (0%, 5%, 10%) with nine treatment combinations. Observation parameters included the percentage of live seedlings, plant height, stem base diameter, and number of active leaves. The results showed that the combination of mycorrhizae and tomato extract significantly affected all growth parameters. The highest treatment (M2E2) resulted in a survival percentage of 99.2%, plant height of 27.2 cm, stem base diameter of 0.95 cm, and the number of active leaves of 7. This increase indicates that mycorrhizae play a role in expanding nutrient uptake and strengthening the root system, while tomato extract provides growth hormones and antioxidants that support the adaptation process. These findings confirm that the combination of 10 g/plant of mycorrhizae and 10% tomato extract is the best treatment for increasing the success of banana plantlet acclimatization effectively, efficiently, and environmentally friendly, and has the potential to be applied to the production of other horticultural seedlings. Keywords: Mycorrhizae, tomato extract, acclimatization, tissue culture, banana plantlets

Item Type:	Article
Subjects:	S Agriculture > SB Plant culture
Divisions:	Fakultas Pertanian > Agroteknologi
Depositing User:	Ikawati Hadi Utami A.Md
Date Deposited:	12 Nov 2025 07:38
Last Modified:	12 Nov 2025 07:38
URI:	http://repository.utp.ac.id/id/eprint/2230

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